- Petunia has been used as research object on flavonoids studies especially anthocyanin biosynthesis. Hf1 gene encode F3\u275\u27H enzyme which plays a role in the synthesis of color in Petunia. Research on the regulation of Petunia color is potential for the development of ornamental plant breeding in Indonesia, hence the information regarding the gene analysis is necessary. Recently, there are many reagent kits for the isolation and purification of plant DNA available, each has different protocols regarding the special character of plant tissue with various secondary metabolites accumulation. Secondary metabolites in plants have different chemical contents, for that reason its needs to develop a proper protocol resulting in pure DNA with high concentrations. This study aims to obtain the effective and efficient DNA isolation and purification protocol for Petunia hybrida. Optimization results showed that samples immersed in absolute ethanol for two days then dried and stored in the freezer until used have given the optimal results for further analysis. The total DNA concentration was 129.3 ng / µl with purity of 1,87.
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机译：-矮牵牛已被用作类黄酮研究的对象，尤其是花色苷的生物合成。 Hf1基因编码F3 \ u275 \ u27H酶，在矮牵牛的颜色合成中发挥作用。矮牵牛颜色调节的研究对于印度尼西亚观赏植物育种的发展具有潜力，因此有关基因分析的信息是必要的。近年来，有许多试剂盒可用于植物DNA的分离和纯化，每种试剂盒针对具有多种次生代谢产物积累的植物组织的特殊特性，都有不同的方案。植物中的次生代谢产物具有不同的化学含量，因此其需要制定适当的规程以产生高浓度的纯DNA。这项研究旨在获得有效和高效的DNA分离和纯化协议的矮牵牛。优化结果表明，将样品浸入无水乙醇中两天，然后干燥并保存在冰箱中直至使用，为进一步分析提供了最佳结果。总DNA浓度为129.3 ng / µl，纯度为1,87。
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